Journal: Frontiers in Oncology
Article Title: Identify and validate RUNX2 and LAMA2 as novel prognostic signatures and correlate with immune infiltrates in bladder cancer
doi: 10.3389/fonc.2023.1191398
Figure Lengend Snippet: LAMA2 and RUNX2 promote the proliferation, migration, and invasiveness, and inhibit apoptosis of BLCA. (A) High expression of LAMA2 and RUNX2 are associated with advanced clinical stages through analyzing GEPIA database. (B) The silencing of LAMA2 and RUNX2 assessed using qRT-PCR. (C) CCK8 assay showing the effects of LAMA2 or RUNX2 knockdown on proliferation of UMUC3 cells. (D) Effects of LAMA2 and RUNX2 on apoptosis of BLCA as determined by flow cytometry. (E) Effects of LAMA2 and RUNX2 knockdown on migration and invasiveness of UMUC3 and 5637 cells, assessed using Transwell assay (** p <0.01, *** p <0.001, ### p <0.001, n = 3).
Article Snippet: In this study, human BLCA cells (UMUC3 and 5637) were obtained from Procell company (Wuhan, Hubei, China).
Techniques: Migration, Expressing, Quantitative RT-PCR, CCK-8 Assay, Knockdown, Flow Cytometry, Transwell Assay